Cayman's EArly Apoptosis Detection Assay Kit employs FITC-conjugated annexin V as a probe for phosphatidylserine (PS) on the outer membrane of apoptotic cells, TMRE as a probe for ΔΨm, DAPI as an indicator of membrane permeability/cell viability, and TO-PRO-3 to indicate the selective permeability of pannexin channels. The EArly Apoptosis Detection Assay Kit allows phenotypic characterization of multiple different cell death parameters at the single-cell level.